Title

Considerations for SARS-CoV-2 Genomic Surveillance

Authoring Organization

Publication Month/Year

October 21, 2024

Last Updated Month/Year

October 28, 2024

Document Type

Consensus

Country of Publication

US

Document Objectives

Next-generation sequencing (NGS) has applications in research, epidemiology, oncology, and infectious disease diagnostics. Wide variability exists in NGS wet laboratory techniques and dry laboratory analytical considerations. Thus, many questions remain unanswered when NGS methods are implemented in laboratories for infectious disease testing. While this review is not intended to answer all questions, the most pressing questions from a public health and clinical hospital-based laboratory perspective will be addressed. The authors of this review are laboratory professionals who perform and interpret SARS-CoV-2 NGS results. Considerations for pre-analytical, analytical, and post-analytical NGS will be explored. This review highlights challenges for molecular laboratory professionals considering adopting or expanding NGS methods.

Inclusion Criteria

Male, Female, Adult, Older adult

Health Care Settings

Laboratory services

Intended Users

Genetics, nurse, nurse practitioner, physician, physician assistant

Scope

Prevention

Diseases/Conditions (MeSH)

D023281 - Genomics, D000086402 - SARS-CoV-2

Keywords

SARS-CoV-2, Public Health Laboratory, Genomic Surveillance, Whole Genome Sequencing

Source Citation

Hirschhorn JW, Babady NE, Bateman A, Blankenship HM, Bard JD, Florek K, Larkin PMK, Rowlinson MC, Wroblewski K, Wolk DM. Considerations for SARS-CoV-2 Genomic Surveillance: A Joint Consensus Recommendation of the Association for Molecular Pathology and Association of Public Health Laboratories. J Mol Diagn. 2024 Oct 21:S1525-1578(24)00249-6. doi: 10.1016/j.jmoldx.2024.09.005. Epub ahead of print. PMID: 39442753.